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Figure 1


Fig. 1. Differential stability of microtubules following anaphase onset. LLC-Pk1 cells stably expressing GFP-tubulin are shown before and at various times after the addition of 33 µM nocodazole. (A) Addition of nocodazole within 2 minutes of anaphase onset induces rapid disassembly of microtubules and prevents cytokinesis. (B) Addition of nocodazole >2 minutes after anaphase onset results in disassembly of the majority of astral microtubules in the polar region and most astral microtubules in the equatorial region, but not of interzonal microtubules. Time is shown in minutes:seconds relative to the addition of nocodazole. Scale bars: 10 µm.





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