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Fig. 4. Actin polymerization and cortical contractions contribute to wave-like behavior of cortical actin in nocodazole-treated anaphase cells. LLC-Pk1 cells expressing GFP-actin were followed until anaphase onset, nocodazole was added and photobleaching performed. Areas enclosed by a red broken line indicate the photobleached regions; the white box indicates the region that is shown in A'; asterisks mark the location of chromosomes; arrows mark the site of cortical ingression. Time is shown in minutes:seconds relative to photobleaching. Scale bars: 10 µm.