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Figure 5


Fig. 5. Mislocalization of TDRFP-MRLC in nocodazole-treated anaphase cells. (A-C) LLC-Pk1 cells stably expressing TDRFP-MRLC. (A) In control cells, TDRFP-MRLC accumulates at the equatorial cortex. (B) In nocodazole-treated cells that fail to assemble a contractile ring, TDRFP-MRLC is diffusely distributed along the ventral cortex. (C) In nocodazole-treated cells that assemble a contractile ring, myosin accumulates at the equatorial cortex and shows transient accumulation in the polar cortex (arrows) (compare the 00:30 time point with 03:50). The first two frames are focused on the accumulation of MRLC at the equator and, as soon as contractile activity is observed away from the ring at 3:50, the focus shifts to the non-equatorial cortex. Time is shown in minutes:seconds after the addition of nocodazole. Asterisks mark the position of the chromosomes. Scale bars: 10 µm.





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