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Fig. 6. Rho is mislocalized and its activity is required for wave-like behavior of cortical actin in nocodazole-treated cells. (A) GFP-CeRhoA is mislocalized to the polar region in nocodazole-treated cells; arrowheads mark an area of GFP-CeRhoA fluorescence in the polar cortex. White boxes indicate the region that is shown to the right. (B,C) LLC-Pk1 cells expressing GFP-actin were treated with nocodazole in anaphase and then injected with C3 transferase (B) or treated with Y-27632 (C). White boxes indicate the region that is shown in B',C'. Addition of either C3 transferase or Y-27632 suppresses wave-like behavior (B',C'); arrows show sites at which cortical actin accumulated during wave-like behavior following treatment with nocodazole. Time of addition of C3 transferase or Y27632 is indicated. Time is shown in minutes:seconds. Scale bars: 10 µm.
