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Figure 10


Fig. 10. Dominant-negative Eps15 does not inhibit fibronectin endocytosis. Dominant-negative Eps15 (A,C,E,G,I,K) or control Eps15 (B,D,F,H,J,L) were transiently expressed in FN-null myofibroblasts. (A-D) Transfected cells were seeded onto preassembled matrices and incubated for 24 hours. Endocytosed fibronectin is shown in A,B. (E-H) Short-term pulse-chase experiments were performed with transfected cells. (E,F) show endocytosed fibronectin following 2 hours of chase. (I-L) The effect of dominant-negative and control Eps15 on transferrin receptor endocytosis is shown. Transfected cells were detected by GFP expression (C,D,G,H,K,L) and manually outlined. 15-20 cells were analyzed for each condition; representative images are shown. Scale bar: 10 µm.





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