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Figure 2


Fig. 2. Colocalization of internalized fibronectin and β1 integrins shortly after initiation of endocytosis. FN-null myofibroblasts were incubated with 10 µg/ml TR-fibronectin and 50 µg/ml FITC-conjugated 9EG7 at 4°C for 1 hour during the pulse. After 30 minutes of chase at 37°C, cells were incubated with 0.2% Trypan Blue for 3 minutes to quench extracellular fluorescence. (A) TR-fibronectin; (B) FITC-conjugated 9EG7; (C) overlay images. Arrowheads in C indicate colocalized TR-Fibronectin and FITC-9EG7. These images are optical sections collected from a confocal microscope. Scale bar: 10 µm.





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