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Figure 7


Fig. 7. Reduced endocytosis and turnover of pre-assembled fibronectin matrix in β1-integrin-null cells. GD25 and GD25 β1 re-expressing cells were seeded on pre-assembled TR (A,B,D,E,F,G) or AF488 (C) fibronectin matrix and incubated for 24 hours at 37°C. (A,B) Cells were incubated with 0.2% Trypan Blue for 3 minutes and then fixed. Intracellular fibronectin vesicles are shown (A, GD25; B, GD25 β1). The insets show outlines of cells loaded with CellTracker Green. Scale bar: 10 µm. (C) Cells were processed for flow cytometry to quantify endocytosed fibronectin. The numbers over the peaks in C are the MFI of internalized AF 488-fibronectin. (D-G) Cells were fixed without Trypan Blue treatment to visualize fibronectin fibrils (D,E, GD25; F,G, GD25 β1; E and G are phase images of corresponding fields). Scale bar: 40 µm.





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