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Figure 1


Fig. 1. Expression analysis of SPR2 and SP2L genes. (A) Structure of Arabidopsis SPR2 and SP2L genes. SPR2 and SP2L consist of four and six exons (shown in thick bars), respectively. The protein-coding regions are in black, whereas the 5'- and 3'-untranslated regions are in white. Tandem copies of GFP were inserted just before the stop codons. PCR amplification results in products with different sizes that arise from cDNAs or genomic regions of SPR2 and SP2L. Mutations in spr2 and sp2l alleles are indicated. (B) Organ-specific expression patterns of SPR2 and SP2L. Various organs of wild-type plants (Col ecotype) were analyzed for expression of SPR2 and SP2L by RT-PCR, using a common primer set. Actin8 was used as a control. The positions of cDNA fragments and genomic fragments are indicated on the left and right sides, respectively. (C) Tissue-specific expression patterns of SPR2 and SP2L. GFP was expressed in the genomic context of SPR2 and SP2L, as indicated in (A). Four-day-old transgenic seedlings were observed by laser scanning confocal microscopy in the same low-power fields and at the equivalent gain levels. Arrows indicate hydathodes and root hairs.





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