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Fig. 4. Microtubule distribution in seedling hypocotyls. Cortical microtubules were visualized by GFP-TUB6 in the upper and lower regions of epidermal cells in 4-day-old and 7-day-old seedlings, respectively. (A) Images from 4-day-old seedlings. (B) Distribution of microtubule orientations. Transverse microtubule orientation is set at 90 degree, whereas orientations in left-handed helical arrays have values less than 90 degrees. The average microtubule angles are indicated with arrows above histograms. Asterisks show statistically significant differences from control (t-test; *P<0.05, **P<0.01).