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Figure 6


Fig. 6. Microtubule plus-end tracking with GFP-EB1. (A,B) Tracks of several GFP-EB1b comets were followed for 5 minutes in hypocotyl epidermal cells of control (A) and spr2-2 (B) plants, and are indicated by colored lines. Circles at one end of GFP-EB1 trajectories show the start points of measurements, whereas arrows indicate abrupt changes in the trajectory. Kymographs are shown for two representative trajectories, in which transient stops of GFP-EB1 are indicated by asterisks. (C) Histograms of GFP-EB1 velocity distribution in hypocotyls and petioles of control and spr2-2 plants. Stationary GFP-EB1 comets with a velocity less than 1 µm/minute are shown in black bars. The stationary comets were excluded when the mean values (± s.d.) of the velocity were calculated. Velocity distributions of spr2 cells and control cells were significantly different (t-test; **P<0.01).





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