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Fig. 7. Parameters of microtubule dynamics in vitro. Dynamic behavior at the plus end of in vitro assembling microtubules was measured at 27°C in the presence of Trigger Factor (TF), TF-SPR2, or TF-SP2L at concentrations of 0.2 µM and 0.4 µM, by using dark field microscopy. (A) Dynamic instability profiles of microtubules at the plus end in the presence of 0.2 µM SPR2 or 0.2 µM SP2L. The pause state is indicated by thick lines. (B) Growth and shrinkage velocities. (C) Transition frequencies among growth (G), shrinkage (S) and pause (P). (D) Total time spent in growth, shrinkage, and pause. (E) Microtubule dynamicity. Data shown are means ± s.d. Statistical significance (t-test; ^*P<0.05 and ^**P<0.01) is shown for TF-SPR2 or TF-SP2L vs TF control at the same concentration. (F) TF-SPR2 increases microtubule polymerization. Turbidity of the 17.5 µM tubulin solution in the presence of 0.5 µM TF (white squares) or 0.5 µM TF-SPR2 (black circles) was monitored at 350 nm and at 37°C. The values are means (± s.d.) from four independent experiments. Sample values; 0.2 µMTF, n=16 microtubules and t=136.9 minutes; 0.4 µMTF, n=12 microtubules and t=130.9 minutes; 0.2 µM TF-SPR2, n=28 microtubules and t=333.1 minutes; 0.4 µM TF-SPR2, n=17 microtubules and t=204.5 minutes; 0.2 µM TF-SP2L, n=20 microtubules and t=245.4 minutes; 0.4 µM TF-SP2L, n=20 microtubules and t=249.8 minutes.

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