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Figure 1


Fig. 1. (A) AFAP-110 localizes to PE-induced podosomes in A7r5 smooth muscle cells. A7r5 cells plated on fibronectin coated glass coverslips were either left untreated (a,b) or were treated with 1 µM PDBu for 1 hour (c,d). Cells were stained with Rhodamine-labeled phalloidin to label F-actin (a,c) and with a polyclonal AFAP-110 antibody (b,d). Confocal microscopy was used to scan 0.7-µm-thick images along the ventral membrane of the cells to image the podosomes. (B) GFP-AFAP-110 was transiently transfected into A7r5 cells and immunofluorescence images were obtained using antibodies to detect endogenous vinculin (b) and cortactin (c), to contrast with GFP fluorescence (a) in the merged image (d). (C) Monomeric dsRed-AFAP-110 was transiently expressed in A7r5 cells that had been plated on an FITC-gelatin matrix containing 50 µg/ml fibronectin.





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