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Figure 6


Fig. 6. Phosphorylated AFAP-110 localizes to podosomes. A7r5 cells were transfected with GFP-AFAP-110 constructs and either left untreated (panels a-d) or treated with 1 µM PDBu for 1 hour (panels e-h). Immunofluorescence staining was performed as described in Materials and Methods, with actin pseudocolored in red, GFP-AFAP-110 in green and the phosphorylation-specific AFAP-110 antibody anti-pSer277 (P-AFAP) in blue. Merged pictures show colocalization of phosphorylated AFAP-110 with AFAP-110 and actin in podosomes.





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