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Figure 7


Fig. 7. GFP-AFAP-110S277A expression is associated with an increased number of cells that contain podosomes. (A) Immunofluorescence staining of A7r5 cells, transfected with wild-type GFP-AFAP-110 and treated with PDBu for 1 hour (A, A', A") to show cellular morphologies demonstrating (A) stress fibers, (A') a mix of stress filaments and podosomes or (A") podosomes only. (B) Quantification of GFP-AFAP-110-expressing or GFP-AFAP-110S277A-expressing A7r5 cells (uninduced and PDBu-treated) that display stress fibers (black), podosomes (white) or a mixed population of stress fibers and podosomes (checkerboard) correlating to A, A" or A', respectively. (C) GFP-AFAP-110S277A expression (pseudocolored green) in A7r5 cells contrasted with TRITC-phalloidin (pseudocolored red) and cortactin detected with monoclonal antibody 4F11 (pseudocolored white). Cells were either untreated or treated with PDBu for 60 minutes. Cells that expressed GFP-AFAP-110S277A expressed either predominantly podosomes or a mix of stress filaments and podosomes.





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