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Fig. 1. Cells on fibronectin-patterned lines are polarized. (A) Patterned, 6-µm thick fibronectin lines and Pll–g-PEG. (B) Bsc1 cells on a standard glass coverslip labelled for the actin network (green), the Golgi complex (red) and the nucleus (blue). (C) Bsc1 cells labelled as in B, grown on 6-µm fibronectin lines. (D) Ptk2 cells labelled as in B, grown on 6-µm fibronectin lines. (E) Centered projection of 100 Bsc1 cells grown on a standard coverslip demarcated to outline the mean cell size and shape, scale bar 10 µm (see supplementary material Fig. S1). (F) Centered and aligned (along the longest cell axis) projection of 100 Bsc1 cells grown on a standard coverslip (see supplementary material Fig. S1). (G) Centered and aligned (along the longest cell axis) projection of 100 Bsc1 cells grown on fibronectin line (see supplementary material Fig. S1). (H) Centered and aligned (along the longest cell axis) overlay of 50 Ptk2 cells grown on a standard coverslip (see supplementary material Fig. S1). (I) Centered and aligned (along the longest cell axis) overlay of 50 Ptk2 cells grown on fibronectin line (see supplementary material Fig. S1). (J) Threshold images of 38 Bsc1 cells grown on 6-µm fibronectin lines, fixed and analysed by immunofluorescence. Actin cell contour (black, phalloidin staining), nucleus (red, Hoechst staining) and Golgi (green, GM130 staining). Scale bars, 20 µm (A-D); 10 µm (E-I).

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