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Figure 5


Fig. 5. The MTOC and the Golgi complex are moved to the new rear upon direction changes. (A-C) Staining of Bsc1 cells for (A) {gamma}-tubulin (centrosomal protein), (B) GM130 (Golgi matrix protein), (C) nucleus (Hoechst staining). (D) Overlay of A-C. (E) Projection of 25 Bsc1 cells as described in supplementary material Fig. S1 for {gamma}-tubulin (red), GM130 (green) and the nucleus (blue). (F) Sequence of the reorientation of a Bsc1 cell. Shown in alternation are transmission images and immunofluorescence images of the GalT-GFP signal (Golgi matrix protein, green) and the {gamma}-tubulin cherry (Gtub-cherry) highlighting the centrosome position (red) taken at 0.5 frames/hour. Direction is indicated by arrows. Centrosome position is highlighted by asterisks.





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