spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. MTS-exchange mutants. (A) Growth and complementation of aconitase and fumarase-MTS-exchange mutants. {Delta}aco1 or {Delta}fum1 strains harboring the indicated plasmids that encode MTS-exchange mutants were diluted and grown on galactose or ethanol-acetate medium plates as indicated (B) Aconitase- and fumarase-MTS-exchange mutants are processed. Wild-type, {Delta}aco1 and {Delta}fum1 strains induced for expression the indicated plasmids were labeled with [35S]methionine for 30 minutes, either in the absence (–) or presence (+) of 20 µM CCCP. Total cell extracts were prepared, immunoprecipitated with the indicated antiserum and analyzed using SDS-PAGE. Arrows show positions of precursor (p) and mature proteins (m). (C) MTS-exchange mutants exhibit alterations in the protein subcellular distribution. Yeast cells expressing aconitase and fumarase variants were subjected to subcellular fractionation. Equivalent portions from the total (Tot) cytosolic (Cyt) and mitochondrial (Mit) fractions were analyzed by western blotting using antibodies against the indicated proteins. Representative examples of mitochondrial (Hsp60) and cytosolic (hexokinase1, HK) controls are shown. Cytosolic and mitochondrial band intensities were quantified densitometrically using TINA Software.





Right arrow Return to article