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Figure 3


Fig. 3. Aconitase-MTS-elongated mutants. (A) Growth and complementation of aconitase-MTS elongated mutants. {Delta}aco1 strains harboring the indicated plasmids encoding MTS elongated mutants were diluted and grown on galactose or ethanol-acetate medium plates as indicated. (B) Aconitase elongated mutants are processed. MTS-elongated mutants of aconitase were labeled and examined for processing as described in the legend to Fig. 1B. (C) Aconitase elongated mutants exhibit alterations in the protein subcellular distribution. Subcellular fractionations of yeast cells expressing aconitase elongated mutants. Equivalent portions from the total (Tot) cytosolic (Cyt) and mitochondrial (Mit) fractions were analyzed by western blotting using antibodies against the indicated proteins.





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