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Figure 5


Fig. 5. The central element is required for the development of meiotic crossovers. (A) Meiotic chromosomes in wild-type and Tex12–/– oocytes immunolabeled for STAG3 (axial elements, green) and DMC1 (red, E16.5 and E18.5), RPA (red, E17.5 and E18.5) or MLH1 (red, E18.5). Centromeres are labeled with CREST (white). Bar: 5 µm. (B) Average number of DMC1 and RPA foci (± s.e.m.) at E16.5 (wt, n=7; –/–, n=4) and E18.5 (wt, n=12; –/–, n=9) and E17.5 (wt, n=6; –/–, n=10) and E18.5 (wt, n=10; –/–, n=9).





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