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Figure 2


Fig. 2. Integrin {alpha}5 is required for efficient RhoA activation and fibronectin fibrillogenesis. (A) Western blot analysis of RhoA activity assay on lysates of EA5 cells expressing indicated integrins. Quantification shows relative RhoA activation ± s.d. compared with EA5 cells from two independent experiments. (B) EA5 cells expressing indicated constructs stained for paxillin (green) and F-actin (red), with the nucleus in blue. Scale bar: 10 µm. (C) Western blot analysis of assembled fibronectin-biotin and vimentin (loading control) in DOC-insoluble lysates of EA5 cells expressing indicated constructs. Locations of molecular size markers (in kDa) are indicated.





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