spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. (A) Mean fluorescence analyzed by flow cytometry, demonstrating binding of integrin β1 and β1-3-1 to different concentrations of soluble FITC-fibronectin. (B) Images of assembled fibronectin-biotin on GE11 cells expressing β1 or β1-3-1; fibronectin is red and nucleus blue. Scale bar: 50 µm. (C) Western blot analysis of RhoA activity assay on lysates of GE11 cells expressing indicated integrins. Quantification shows relative RhoA activation ± s.d. compared with GEβ1 cells from two independent experiments. (D) Images of GE11 cells expressing β1 integrin or β1-3-1 stained for paxillin (green) and F-actin (red). Scale bar: 10 µm.





Right arrow Return to article