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Figure 7


Fig. 7. Soluble fibronectin binding to {alpha}5β1 integrin supports Rho GTP-loading independent of ROCK or myosin-II activity or fibronectin fibrillogenesis. (A) Images of assembled fibronectin-biotin on GEβ1 cells treated with inhibitors of ROCK (Y-27632, 10 µM) or myosin-II (Blebbistatin, 50 µM) or with DMSO (control); fibronectin is stained red and nucleus blue. Scale bar: 50 µm. (B) Western blot analysis of RhoA activity assay on lysates of GE11 cells treated with the indicated inhibitors. Quantification shows relative RhoA activation ± s.d. compared with GEβ1 cells from three independent experiments. (C) Binding of soluble fibronectin-biotin (10 µg/ml) to GEβ1 cells treated with the indicated inhibitors.





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