spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Syndecan-4 is not required for {alpha}5β1-integrin-supported RhoA signaling. (A) Flow cytometry analysis of endogenous syndecan-4 surface expression on GEβ1 cells transfected with syndecan-4 or control siRNA. (B) Images of GEβ1 cells transfected with syndecan-4 or control siRNA stained for paxillin (green), F-actin (red) and the nucleus (blue). (C) Flow cytometry analysis of soluble FITC-fibronectin (10 µg/ml) binding to GEβ1 cells with or without syndecan-4 knockdown. (D) Images of assembled fibronectin-biotin on GEβ1 transfected with syndecan-4 or control siRNA; fibronectin is red and nucleus is blue. Scale bar: 50 µm. (E) Western blot analysis of RhoA activity assay on lysates of syndecan-4 knockdown and control GEβ1 cells. (F) Flow cytometry analysis of endogenous syndecan-4 surface expression on GEβ3 cells transfected with syndecan-4 or control siRNA. (G) Images of GEβ3 cells transfected with syndecan-4 or control siRNA stained for paxillin (green) and F-actin (red).





Right arrow Return to article