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Fig. 4 The Lsm4 C-terminus is required for efficient mRNA degradation, but not splicing. (A) Degradation of PGK1pGmini reporter transcript in LSM4 (MRY71) or lsm4C (MRY73) strains grown in SDGal-Ura after addition of glucose to 4% (w/v). scR1 RNA was used as a loading control. The asterisk indicates a stable degradation fragment. (B) PGK1pGmini transcript levels over time as a percentage of the level in LSM4 cells at t=0; averages of three northern blots with vertical bars indicating standard deviations. (C) Linearized degradation curves [–ln(PGK1_t/PGK1_t=0)] against time showing averages of qRT-PCR data of six RT repeats of two independent biological replicates; vertical bars indicate standard errors; half-lives indicated are based on the linearized qRT-PCR data (D) Northern blot detecting pre-U3 RNA and U3 RNA in LSM4 and lsm4C strains grown in YPDA, and in a P_GAL-LSM8 strain (MPS7) before and after 12 hours of growth on glucose.

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