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Fig. 3. The concentration of extracellular CfaD increases with cell density. (A) SDS-polyacrylamide gel of the rCfaD used for the calibration curve was stained with Coomassie Blue. Molecular mass markers (in kDa) are shown at left. (B) The amount of CfaD as a function of cell density was determined by staining western blots of conditioned growth medium from wild-type cells at the indicated densities together with standard amounts of rCfaD with affinity-purified anti-CfaD antibodies. Values are the mean ± s.e.m. from three independent experiments. The absence of error bars indicates that the error was smaller than the plot symbol. The insert shows a western blot of conditioned growth media harvested at the indicated densities (in units of 106 cells/ml) stained with affinity-purified anti-CfaD antibodies. Molecular mass markers (in kDa) are at left.
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