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Figure 4


Fig. 4. Size fractionation of complexes containing CfaD and AprA. Conditioned starvation medium (CSM) and conditioned growth medium (CGM) from the indicated cells were concentrated, and were then fractionated using molecular-sieve chromatography. WT, wild type. Western blots of the different fractions were stained with affinity-purified anti-CfaD and anti-AprA antibodies. At the top, numbers indicate fraction number, and the position of molecular-sieve molecular-mass markers is indicated. A 670 kDa marker eluted at fraction 30. For each of the western blots, the position of molecular mass markers is indicated at left.





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