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Fig. 3. Suppression of aPKC activity does not disrupt the polarized organization of vinculin-containing structures at regions of cell-cell and cell-substrate contact. (A) MTD1-A cells treated with the indicated adenovirus vectors or myosin inhibitors were subjected to a Ca²⁺ switch as described in the legend for Fig. 1, and were then immunostained with rhodamine-phalloidin (top panels) and an anti-vinculin antibody (bottom panels) at 6 hours after the Ca²⁺ switch. Single confocal sections at the basal planes (basal) and apical planes 2.5 µm upward from the basal planes (apical) are shown. The signal intensity of phalloidin staining in the basal planes was enhanced to a comparable level to that in the apical planes. Note that aPKC kn does not disrupt the polarized distribution of vinculin into the two distinct structures at apical cell-cell contacts (arrows) and basal cell-substrate contacts (arrowheads). By contrast, cells treated with myosin-II inhibitors exhibit vinculin staining in the same planes. (B) Reconstituted xz views of the cells demonstrated in A were presented for rhodamine-phalloidin staining. Arrowheads point to the apical and basal planes at which the confocal data were obtained. Scale bar: 10 µm.

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