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Figure 7


Fig. 7. aPKC{lambda} kn does not affect the formation of the radial actin fibers that is rapidly induced by LPA in serum-depleted MTD1-A cells. (A) Subconfluent MTD1-A cells expressing GFP-actin together with β-galactosidase (LacZ) or aPKC{lambda} kn were depolarized in LC medium for 48 hours and subjected to serum starvation as described in the legend for Fig. 4. CaCl2 and LPA were simultaneously added to the cells at time 0, and time-lapse images were acquired by confocal microscopy (see supplementary material Movies 4, 5). The images shown are still-frames (projected xy views of intermediate sections) from the time-lapse data at the indicated times. (B) Enlarged views corresponding to the boxed regions in A. Scale bar: 10 µm.





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