Laminin 5 influences the architecture of the mouse small intestine mucosa

JCS025528 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of laminin α chains in the colon. Mouse colon sections were stained with antisera directed against laminin α chains, as indicated. (A-C) There were no differences in expression between Het/Tg colon and WT colon. The expression of laminin α5 in the KO/Tg colon was reduced compared with controls. (D-F) The expression of laminin α1 in the KO/Tg colon was upregulated compared with controls. (G-I) There were no significant differences in laminin-α4 staining between KO/Tg colon and controls. Bars, 100 µm.

• Supplemental Figure S2 -

  Fig. S2. (A) Whole-mount views of mouse intestines, mucosa side up. Villus coalescence was observed throughout the KO/Tg small intestine (second row), with distal small intestine being the most severely affected segment. PSI, proximal small intestine; MSI, middle small intestine; DSI, distal small intestine. (B) There were no differences in morphology among WT, Het/Tg, and KO/Tg colons.

• Supplemental Figure S3 -

  Fig. S3. Sections of embryonic intestinal grafts were stained with antiserum directed against laminin-α chains, as indicated. Levels of laminin-α1, -α2 and -α4 were elevated in the subepithelial BMs of Lama5^−/− small intestine (E,H,K). Compared with the control small intestine graft (A,D,J), control colon graft contained higher levels of laminin α1 and laminin α4, but lower levels of laminin α5 (C,F,L). Arrowheads in J, K, and L denote the position of subepithelial BMs, seen as linear staining for laminin α4 in K and L.

• Supplemental Figure S4 -

  Fig. S4. Sections of distal small intestines from Het/Tg and KO/Tg mice were stained with antibodies against chromogranin A to mark enteroendocrine cells (A,B), with antibodies against lysozyme to mark Paneth cells (C,D), and with periodic acid-Schiff to mark goblet cells (E,F). The number of enteroendocrine cells was comparable between Het/Tg and KO/Tg small intestine. There were increased numbers of Paneth cells and goblet cells in the KO/Tg small intestine compared with Het/Tg small intestine. Bars, 100 µm.

• Supplemental Figure S5 -

  Fig. S5. Sections of distal small intestines from Het/Tg and KO/Tg mice were stained with anti-integrin α3 (A,B), integrin α6 (C,D), integrin β1 (E,F), integrin β4 (G,H), dystroglycan (I, J), and activated integrin β1 (K,L). The localization and expression levels of these receptors were comparable between Het/Tg and KO/Tg mice. Bars, 100 µm.