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Figure 1


Fig. 1. Real-time measurement of anaphase kinetics. (A,B) Representative time-lapse sequences (2D projection, combined DIC and green-fluorescence images) of spindle elongation followed by Nuf2-GFP fluorescence. The different anaphase-B phases (fast phase, pause phase and slow phase) are indicated at the bottom of the frames. (A) kip1{Delta} cells; (B) kip1{Delta} cin8-FA cells. The time at which each frame was taken is indicated. Scale bar: 2 µm. (C) Representative real-time spindle-elongation kinetics in cells expressing kinesin-5 mutants. Cells were arrested in S-phase of the cell cycle for 2 hours and then released from arrest. The distance between spindle pole bodies (SPBs), monitored by Nuf2-GFP fluorescence, was measured as a function of time. Time of initiation of spindle elongation was assigned as 0 minutes. `R' represents the rate of spindle elongation. The different anaphase-B phases are indicated as follows: fast phase, diamond; pause, square; slow phase, triangle; post-anaphase spindle elongation or spindle collapse, circle. The genotypes are indicated at the bottom of each plot: (a) wild type; (b) kip1{Delta}; (c) cin8-FA; (d,e) kip1{Delta} cin8-FA.





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