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Figure 2


Fig. 2. (A-D) Anaphase spindle morphologies in WT (A), kip1{Delta} (B) cin8-FA (C) and kip1{Delta} cin8-FA (D) cells. Cells expressing tubulin-GFP ware examined by high-resolution fluorescence microscopy (see Materials and Methods). Representative 2D images are shown. Spindle length (µm) is indicated at the bottom of each spindle image. Normal, asymmetric and indistinguishable midzone (ind. midzone) morphologies are indicated at the top. Arrows indicate the location of the midzone region. (E) Percentage of spindles with defects. Left, percentage of intermediate (3-5 µm) asymmetric spindles; right, percentage of anaphase spindles with an indistinguishable midzone region. For each genotype, 80-120 anaphase (>3 µm) spindles were analyzed, half of which were of intermediate length. (F,G) Spindle localization of Ase1 and Slk19 proteins. (G) Images of unsynchronized cells expressing either Ase1-GFP (left) or Slk19-GFP (right) were acquired during anaphase. Representative images (2D projection) of spindles are shown. SPBs are labelled with Nuf2-GFP. Spindle length (µm) is indicated on the bottom of each image. (F) Length of Ase1-GFP signal (left) and Slk19-GFP signal (right), expressed as a percentage of spindle length in intermediate (3-5 µm) and long (>5 µm) spindles. Average and s.d. of 25-35 measurements are shown. Genotypes are as indicated: kip1{Delta}, white; kip1{Delta} cin8-FA, grey.





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