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Figure 3


Fig. 3. Effect of SLK19 or SPO12 chromosomal deletion on spindle and nuclear morphology (A), and on cell viability (B). (A) Cells were arrested in S-phase of the cell cycle by hydroxyurea, and were processed for DNA and tubulin immunostaining following release. Representative DNA (left) and spindle (right) morphologies of cells with intermediate or long (4-7 µm) spindles are shown. Genotypes are indicated at the bottom of each panel. Scale bar: 4 µm. (B) Cells were grown overnight, diluted to equal density, plated in serial dilutions and grown at 26°C (left) or 33°C (right) for 3 days. Genotypes are indicated on the left.





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