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Figure 5


Fig. 5. (A) Effect of the overexpression of Cdc20 on spindle elongation kinetics in kip1{Delta} cin8-FA cells. Cells either carrying plasmid containing CDC20 under the control of the MET25 promoter (PMET->CDC20; a,b) or vector only (c,d) were grown to log phase in medium containing methionine. To induce Cdc20 overexpression, cells were placed into methionine-lacking medium (– methionine; b,d). The distance between SPBs, monitored by Nuf2-GFP fluorescence, was measured as a function of time. `R' represents the rate of spindle elongation. The different anaphase-B phases are indicated by the following symbols: fast phase, diamond; pause, square; slow phase, triangle; prior to spindle elongation, post-anaphase spindle elongation or spindle collapse, circle. (B) Representative anaphase DNA morphologies in kip1{Delta} cin8-FA cells overexpressing Cdc20. Normal and deformed DNA morphologies are shown. Arrows point to lagging DNA in cells with two DNA masses separated by more than 5 µm. Scale bar: 5 µm. (C) Percentage of cells with all DNA deformations (left) and percentage of cells with two deformed DNA masses separated by more than 5 µm (right) in anaphase cells. In each genotype, indicated at the bottom, 200-220 cells were analyzed.





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