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Figure 3


Fig. 3. FRET analyses of core-localizing NE proteins. (A-H) HeLa cells transiently expressing two fusion proteins, as indicated, were analyzed by the acceptor photobleaching method: FRET signals were detected by an increase of donor fluorescence after photobleaching the acceptor chomophore. (A,C,E,G) Typical examples of images at 480 nm for mCFP and 520 nm for mVenus. The bleached area is indicated by the white square; the inset in the upper right corner is an enlarged image of the bleached area. (B,D,F,H) Fluorescence spectra measured in the bleached area before and after acceptor photobleaching. (I) HeLa cells transiently expressing CFP-BAF and YFP-BAF were analyzed by the ratio-imaging method. Images were obtained every minute. Time 0 represents timing of the metaphase-anaphase transition. Ratio images are represented by the color code shown on the right. Scale bars:10 µm (A,C,E,G).





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