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Figure 4


Fig. 4. Live-correlated EM analyses. (A) Time-lapse images of living HeLa cells expressing GFP-BAF were obtained every minute. Magenta represents chromosomes (Hoechst 33342) and green represents GFP-BAF. Cells were fixed after live-cell fluorescence imaging, and the same cell was subjected to EM analysis. (B) A series of deconvolved 3D images of the fixed cell. (C) A single section image from the indicated panel in B. (D) An EM image corresponding to the image in C. (E) Magnified view of the region indicated in D. (F) High-magnification EM images of the regions a-c indicated in E. In the lower panels, drawings are superimposed on the EM images indicating GFP-BAF (green), the NE (red), MTs (orange) and vesicles (purple arrowheads). Scale bars: 10 µm (A,C), 2 µm (D) and 200 nm (F).





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