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Figure 9


Fig. 9. Live-correlated EM analyses of HeLa cells expressing GFP-BAF after siRNA treatment (D-H) or luciferase siRNA treatment as a control (A-C). During time-lapse imaging of living HeLa cells expressing GFP-BAF, cells were fixed 7 minutes after the metaphase-anaphase transition and subjected to EM analysis. A magnified view of the region indicated in A is shown in B and C. Magnified views of the regions indicated in D are shown in E and F (left square region), and G and H (right square region). In C, F and H, drawings are superimposed on the EM images indicating GFP-BAF (green), the NE (red), and MTs (orange). Scale bars: 2 µm (A,D), 500 nm (B,C,E-H). (I) Model of NE formation in the control siRNA-treated (left) and BAF siRNA-treated cell (right). For details, see text.





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