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Figure 3


Fig. 3. Expression changes of macrophage surface markers by JNK-inhibitor treatment. BMMs were cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for 2 days to generate pOCs. pOCs were further incubated with M-CSF and RANKL for 24 hours in the absence (–SP) or presence (+SP) of SP600125. (A-D) Cells were stained with fluorescein-conjugated anti-CD11b (A), -CD68 (B), -F4/80 (C) or CD14 (D) as surface markers of macrophages and/or monocytes, and were then subjected to flow-cytometry analyses. The x-axes represent fluorescence intensity for FITC-zymosan-positive cells. Bar graphs show the percentage of cells positive for each surface marker. (E) Histograms represent the MFI of the stained cells. Similar results were observed in another experiment.





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