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Figure 8


Fig. 8. Modulation of NFATc1 level by CaMK in pOCs. (A) Mouse BMM-derived pOCs were transfected with oligonucleotides for CamKII{delta} and/or CamKIV siRNA, and the protein level of NFATc1 was analyzed by western blotting. (B-D) pOCs were treated for 24 hours with or without KN93 at the indicated concentrations in the presence of RANKL and M-CSF. NFATc1 protein level (B), the percentage of TRAP+ cells (C) and phagocytic activity (D) were determined. (C,D) Error bars represent s.d. from triplicate samples. Results are representative of three independent experiments. txt, treatment.





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