|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 12. MO targeting Trx reduces neurite outgrowth from dissociated DRG neurons. (A-H), dissociated DRG neurons nucleofected with carboxyfluorescein-labelled β-globin MO. Images shown in A, B or C represent the same field of cells viewed using FITC, TRITC filters or phase contrast, respectively. (A) Most of the cells are carboxyfluorescein-positive and, although signal has preferentially accumulated in the nucleus, extensive fluorescence is also associated with the cytoplasm. (B) Non-specific labelling with TRITC-conjugated anti-rabbit IgG (in the absence of primary antibody), serving as a control for (D) which is immunostained for Trx. (E) is a phase-contrast image of (D). (F), β-globin MO nucleoporated DRG neurons immunostained for Trx. (G,H), lower magnification images labelled with Calcein of freshly dissected (G) or conditioned (H) DRG neurons. (I-K) Images equivalent to those shown in F-H, but neurons were electroporated with a Trx MO. Note that immunofluorescence of Trx is reduced in the Trx MO-treated neurons and also that in this latter case the extent of neurite outgrowth is significantly reduced in freshly dissociated (but not conditioned) neurons. Scale bars, 50 µm. Magnifications of A-F and I, G and J, H and K are identical.
|
