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Figure 2


Fig. 2. Effects of knockdown of AR or Skp2 on the proliferation and differentiation of LNCaP cells in FBS media. (A) LNCaP cells were transduced with the indicated lentiviral constructs for 2 days and used for the cell-proliferation assay (see Materials and Methods). Relative cell number at the end of a 12-day culture in FBS-media are presented. (B) Western blots of LNCaP cells transduced with the indicated lentiviral constructs (top) for 3 days. (C) Phase-contrast photographs of cells shown in A before harvesting for the cell-proliferation assay. Results shown are representative of at least three independent experiments with LNCaP cells at various passages.





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