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Fig. 4. Regulation of Skp2 protein levels in LNCaP cells by androgen. Androgen regulates Skp2 protein levels in LNCaP cells. (A) Proliferation of LNCaP cells, measured 12 days after culture in the indicated media. (B) Western blots of LNCaP cells cultured in the indicated media for 2 days at about 70% confluency. (C) Time-course experiments with LNCaP cells. Cells were incubated in CDT media for 2 days before the start of this experiment. Cells were harvested at the indicated time points for western blot analyses.