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Figure 5


Fig. 5. Regulation of Skp2 mRNA and protein stability of androgen. Androgen regulates Skp2 mRNA levels and protein stability. (A) Western blots of LNCaP cells cultured in the indicated conditions. (B) Semi-quantitative RT-PCR of LNCaP cells cultured in the indicated conditions. (C) Western blots of LNCaP cells incubated in the indicated conditions for 3 days [treatment of MG-132 (50 µM) was for the last 16 hours]. (D) CHX treatment and chase to determine the degradation of Skp2 and Cdk2 after LNCaP cells were transferred from FBS media to either CDT or CDT+R1881 media. Western blot films were scanned, quantified and plotted. All results are representative of three independent experiments.





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