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Figure 7


Fig. 7. Inhibition of cell proliferation and induction of CMV-expressed FLAG-Skp2 protein accumulation by high concentrations of androgen. High concentrations of androgen inhibit cell proliferation and induce accumulation of CMV-expressed FLAG-Skp2. (A) BrdU labeling of LN-FLAG-Skp2 cells cultured in various concentrations of R1881 as indicated (20p, 20 pM; 2n, 2 nM). (B) Western blots of LN-FLAG-Skp2 cells cultured at the indicated conditions for 3 days. (C) CHX experiment as in Fig. 5D. (D) LN-FLAG-Skp2 cells were transduced with lentiviruses expressing control or AR miRNA and treated with various concentrations of R1881 to determine their effects on FLAG-Skp2 protein levels as in B. These results are representative of three independent experiments. (E) LAPC4 and DU145 prostate-cancer cell lines were similarly treated with increasing concentrations of R1881 for 3 days and subjected to western blot analysis. These experiments were performed twice with identical results.





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