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Figure 9


Fig. 9. Green-fluorescence flow-cytometry analysis of the effects of androgen. (A) LNCaP cells transduced with lentiviruses expressing the indicated proteins (top) were incubated in CDT or CDT+R1881 (100 pM) for 3 days. Cells were trypsinized, resuspended in PBS and used immediately for flow-cytometry analysis. Twenty thousand cells were counted. The position of uninfected (GFP negative) cells in the same cell cultures was representatively indicated by a filled arrow in the top-left FACS plot. (B) LNCaP cells expressing NGFP were subjected to the same analysis, except that a serum-free-medium condition was included. All results are representative of three independent experiments.





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