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Figure 6


Fig. 6. Mitochondrial tubular networks are maintained in {rho}0 HeLa cells. (A) Total cell lysates from wild-type or {rho}0 HeLa cells were analyzed by immunoblotting using antibodies targeting the indicated proteins. {rho}0 HeLa cells completely lost the supercomplexes. (B) Mitochondrial fractions from wild-type or {rho}0 HeLa cells were digitonin-solubilized, subjected to Blue-Native gel electrophoresis, and immunoblotted with antibodies against the indicated proteins. Arrowhead indicates the supercomplexes of complexes I, III and IV. (C) The {rho}0 HeLa cells transfected with the plasmid carrying Su9-DsRed were analyzed by confocal microscopy. {rho}0 HeLa cells maintain mitochondrial tubules, which are not expanded. Scale bar, 10 µm. (D) The {rho}0 HeLa cells were fixed, and thin sections were visualized by electron microscopy. Arrowheads indicate mitochondria. Scale bar, 950 nm.





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