spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Thr1590 plays an essential role in the effects of Kalirin-7 on PC12 cell morphology. (A) PC12 cells expressing Kalirin-7 T/A or Kalirin-7 T/D were examined 24 hours post-transfection. Kalirin-7 was visualized using antibody to Myc (green) and filamentous actin was visualized using TRITC-phalloidin (red). Arrowhead indicates protrusion, stars indicate nontransfected cells, arrows indicate nuclei. (B) Cell perimeter and roundness were quantified as described in Fig. 1: GFP, n=27; Kalirin-7 (Kal7), n=24; Kalirin-7-T/A (Kal7 T/A), n=15; Kalirin-7-T/D (Kal7 T/D), n=24. (C) PC12 cells cotransfected with vectors encoding GFP-DN Cdk5 and either Kalirin-7 T/A or Kalirin-7 T/D were fixed after 24 hours. Exogenous Kalirin was visualized with Myc antibody (blue); DN Cdk5 was identified based on coexpression of GFP; filamentous actin was visualized with TRITC-phalloidin (red). Arrowhead, protrusion; white stars, nontransfected cells; arrows, nuclei. (D) Cell perimeter and cell roundness were evaluated as described in Fig. 1: control GFP, n=18; Kalirin-7-T/D, n=15; Kalirin-7-T/D with DN Cdk5, n=18. P values were calculated using Student's t-test (two-tailed) and bars indicate s.e.m.; #P<0.001; *P<0.05. Scale bars, 10 µm.





Right arrow Return to article