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Figure 6


Fig. 6. Protein phosphatase 1 rapidly dephosphorylates Kalirin phosphorylated on Thr1590. (A) pEAK RAPID cells transiently transfected with vector encoding Kalirin-7 were treated with phosphatase inhibitors as listed on right for 30 minutes before extraction in SDS lysis buffer. Duplicate aliquots of cell extract were visualized with antibody to Thr1590-P (upper panel) or Myc (lower panel). (B) pEAK RAPID cells transiently transfected with parent vector or vectors encoding Kalirin-7, {Delta}Kalirin-7 or KGEF1 were treated with or without 25 nM calyculin A for 30 minutes before extraction into SDS lysis buffer. Duplicates of cell extracts were visualized with antibody to Thr1590-P (P-T1590 upper panel) or Myc (lower panel). (C) PC12 cells expressing exogenous Myc Kalirin-7 and striatal cultures expressing endogenous Kalirin-7 were exposed to medium containing 10 µM roscovitine for 4 hours or 25nM calyculin A for 30 minutes (CA); control cells (Con) received vehicle.





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