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Figure 9


Fig. 9. Kalirin-7 T/D shows increased solubility compared with Kalirin-7. (A) Cortex from adult rat brain was separated into cytosol, organelle and TX-100-insoluble fractions; 20 µg protein from each fraction was subjected to western blot analysis using antibody specific for Kalirin-7. (B) PC12 cells expressing wild-type Kalirin-7, Kalirin-7 T/A and Kalirin-7 T/D were separated into soluble and insoluble fractions; 10% of the soluble fraction (Sol) and 1% of the resuspended pellet fraction (Pel) was subjected to western blot analysis for Myc. Bar graph shows quantification of data from two independent experiments; error bars indicate range.





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