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Figure 5


Fig. 5. Both CG10806 and CG31052 localise to the apical plasma membrane. Flies transgenic for UAS-CG10806::eYFP or UAS-CG31052::eYFP fusions were crossed to GAL4 line c42 to drive expression in the Malpighian tubule principal cells, and subcellular localisation in 1-week-old adult progeny was established by confocal microscopy. DAPI was used to visualise the nuclei (blue) to demonstrate the apical localisation of the transgenic proteins. (A) CG10806 transcript A; (B) CG10806 transcript B; (C) CG31052. (D,E) For comparison, a vha55::GFP transgene marking the apical membrane (D) and immunocytochemistry against the Na+-K+ ATPase {alpha} subunit (a marker of the basolateral membrane), together with GFP-tagged vhaSFD to mark the apical membrane (E) are also shown. Lu, apical tubule lumen; AM, apical plasma membrane; BM, basal plasma membrane. Scale bars: 10 µm.





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