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Figure 7


Fig. 7. Overexpression of CG10806 but not CG31052 in tubule principal cells confers a fluid-secretion phenotype. Basal and neuropeptide-stimulated fluid-secretion rates were measured in c42>CG10806-RB::eYFP (A) or c42>CG31052::eYFP (B) Malpighian tubules and compared to the UAS parents. Basal secretion rates were assessed for 30 minutes before tubules were challenged with the diuretic peptide Capa-1 (100 nM). Secretion rates were then measured for a further 1 hour. (C) As in A, but tubules were stimulated with 1 µM cGMP at the time shown. n=9-12 for each experiment; error bars represent the s.e.m.





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